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ECL
ECL Western Blotting Systems
Selection Guide
ECL System Overview
Technical Specifications
System Components
Results Comparison

ECL Plex
ECL Advance
ECL Plus
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The power of ECL™ Systems


ECL Plex™— multiplex detection on multipurpose imagers
  • Optimized system for quantitative analysis
  • CyDye™ fluorescent technology enables multiwavelength detection
  • No need to strip and reprobe blots, reducing variability
  • Measures significant differences in protein levels with high specificity
  • Highest sensitivity with multiplexing (~1 pg in model system)
ECL Advance™— minimizes the use of valuable antibody
  • Primary antibody concentrations as low as 1 : 100 000
  • Maximum signal-to-noise ratio
  • Demonstrably superior to other high-sensitivity reagents
  • Highest sensitivity with minimum amount of antibody (~ 1 pg in model system, equivalent to ECL Plex)
ECL Plus™— chemiluminescent and chemifluorescent detection
  • Higher sensitivity (~5 pg in model system using film, ~40 pg using imager)
  • Intense light output at 440 nm
  • Extended signal duration
  • Quantitative detection on a fluorescent scanner
ECL™— most cited chemiluminescent reagent for Western blotting
  • Excellent reagent for high-sensitivity detection
  • 10-fold more sensitive than colorimetric methods
  • High sensitivity (~10 pg in model system)