Hydrophobic interaction chromatography (HIC)

Hydrophobic interaction chromatography is well-established in process-scale protein purification today. Its selectivity is complementary to ion exchange and gel filtration (size exclusion chromatography), which together with its ability to clear endotoxins, nucleic acids and viruses, makes the technique a powerful tool for purifying therapeutic proteins.


Selection Kits - Ideal tools for small-scale media screening and selection in industrial process development.

HiTrap™ HIC Selection Kit


For capture and intermediate purification. Extensively used and well-proven in validated large-scale biopharmaceutical production:

Butyl-S Sepharose™ 6 Fast Flow


Butyl Sepharose 4 Fast Flow


Octyl Sepharose 4 Fast Flow


Phenyl Sepharose 6 Fast Flow (high sub) and Phenyl Sepharose 6 Fast Flow (low sub)


For intermediate purification and polishing with high resolution:

Butyl Sepharose High Performance


Phenyl Sepharose High Performance