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Affinity chromatography - A technique in which a biospecific adsorbent is prepared by coupling a specific ligand (such as an protein, peptide or nickel) for the molecule of interest to a solid support.
The inherent high specificity of ligand/target interactions makes affinity chromatography particularly suitable for the capture stage of downstream processing. The result is reliable product purity, often with purification factors well over 1000, and effective concentration achieved in a single step. Affinity chromatography may also be suitable for the intermediate or polishing stages, to remove small amounts of specific contaminants.
GE Healthcare has developed a wide range of affinity chromatography media. Protein A chromatography media are the well-established industrial standard for purification of monoclonal antibodies. Other group-specific ligands such as heparin, chelated metals or dyes are used in a wide range of applications. Pre-activated media enable successful and convenient immobilization of ligands appropriate to your specific application. The inherent high specificity of ligand/target interactions makes affinity chromatography particularly suitable for the capture stage of downstream processing. The result is reliable product purity, often with purification factors well over 1000, and effective concentration achieved in a single step. Affinity chromatography may also be suitable for the intermediate or polishing stages, to remove small amounts of specific contaminants.
Read more about the basic principles behind affinity chromatography.
Read about our Protein A -, Protein G - , IMAC - , Heparin - , dye -, pre-activated -, or other affinity chromatography media.
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