Feedstream with | Pre-treatment technique |
| Total cells / Cell debris | Evaluate
- Centrifugal separator
- Filtration aids (microfiltration, depth filtration, dead-end filtration etc.)
- Aquenous two-phase systems
- Expanded bed adsorption chromatography for capture
|
| Large volume (or low expression yield) | Evaluate
- Ultrafiltration techniques (1-750 kDa exclusion limit)
- Partitioning by aqueous two-phase systems
- Avoid precipitation techniques
|
| High viscosity (>4cP) | Evaluate
- Diafiltration if caused by small molecules like sugars etc.
- Dilution, if sample volume is small
- Enzymatic treatment, if caused by large molecules like DNA
- Expanded bed adsorption chromatography for capture
|
| High conductivity (>5mS/cm) | Evaluate
- Diafiltration in case of large sample volumes
- Dilution, if sample volume is small
- Hydrophobic interaction chromatography for capture, alternatively
|
| Additives incompatible with chromatography | Evaluate
- Ultrafiltration or diafiltration in order to remove additives like solubilizers, anti-foam chemicals etc.
|
