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This handbook is intended to provide guidelines for performing high-resolution two-dimensional (2-D) electrophoresis. It is divided into seven chapters: Chapter 1 provides guidelines for sample ...
This handbook describes the role of affinity chromatography in the purification of biomolecules, the principle of the technique, the media available and how to select them, application examples and ...
This handbook provides technical information and basic operating instructions for the ÄKTAcrossflow system. In addition, maintenance schedules, instructions for troubleshooting and user maintenance ...
This handbook is intended to help you make full use of your ÄKTAexplorer™ or ÄKTApurifier™ chromatography system. Rather than being a text book in separation science, the handbook is instead a ...
This Safety Handbook is valid for all variants of standard ÄKTAprocess™ systems. The purpose of this Safety Handbook is to describe safety precautions and emergency procedures for ÄKTAprocess ...
The purpose of this handbook is to present the most effective and most frequently used strategies for sample preparation and purification of the many different forms of antibodies and antibody ...
Array-based genetic analyses start with cDNAs or oligonucleotides, immobilized on a substrate. The array elements are hybridized with a single labeled sequence, or a labeled complex mixture derived ...
DNA silver staining, 1 mm thick unbacked gels and 0.5 mm thick gels on plastic backing Silver staining is the most sensitive method for permanent staining of proteins or nucleic acids in ...
The new Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both ...
Follow the disassembly/assembly instructions in the document Monitor ALP-900 Instructions (28-4047-75 Edition AD) when performing maintenance on the air trap. After assembly, the air trap should be ...
The new Hoefer Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both ...
This manual provides the basic methodology for making and using gradients of Percoll and includes information on Percoll PLUS, a silica based colloidal medium optimized for cell separation in ...
Fluorescent labelling and staining, when combined with an appropriate imaging instrument, is a sensitive and quantitative method that is widely used in molecular biology and biochemistry laboratories ...
Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. ...
This handbook describes the Glutathione S-transferase (GST) Gene Fusion System which is a versatile system for the expression, purification and detection of fusion proteins produced in Escherichia ...
Isoelectric focusing methods are widely applied for the separation of proteins, peptides and enzymes. The principle: In a pH gradient the sample components migrate towards the anode or the cathode to ...
This handbook describes both theoretical and practical aspects of the technique, the media available and how to select them, together with application examples and detailed instructions for the most ...
IEX for the separation of biomolecules was introduced in the 1960s and continues to play a major role in the separation and purification of biomolecules. Today, IEX is one of the most requently ...
This handbook describes the methodology for making gradients of Ficoll-Paque™ media for density centrifugation of human mononuclear cells. Methodology and applications of Ficoll-Paque PLUS, a ...
Microarray analysis has emerged in the last few years as a flexible method for analyzing large numbers of nucleic acid fragments in parallel. Its origins can be traced to several different ...
Cell culture techniques have become vital to the study of animal cell structure, function and differentiation, and for the production of many important biological materials such as vaccines, enzymes, ...
Polymerase chain reaction (PCR) has revolutionized the field of molecular biology due to its exceptional speed, specificity, and sensitivity as a tool for genetic analysis. Of note also is the ...
This Technical Manual contains information that has been distilled from more than 30 years of laboratory experience in developing and verifying applications and product performance for protein ...
The development of techniques and methods for protein purification has been an essential pre-requisite for many of the advancements made in biotechnology. The key to successful and efficient protein ...
This handbook is intended for students and experienced researchers with an interest in the isolation of integral membrane proteins, multiprotein complexes, or in refolding proteins from inclusion ...
The purpose of this manual is to assist users of Biochrom Ltd UV/Visible Spectrophotometers in the qualification of their instruments in accordance with the
European Pharmacopoeia, Supplement 2000. ...
Amersham Biosciences has more than fifty years of experience in the manufacture and supply of radiochemicals. A continuing programme of investigation of the self-decomposition of radiochemicals on ...
This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of ...
This review is intended to guide readers to the most appropriate exposure conditions for optimal sensitivity, resolution or quantitative accuracy when detecting radioisotopes by radiographic film or ...
This review is intended to guide readers to the most appropriate exposure conditions for optimal sensitivity, resolution or quantitative accuracy when detecting radioisotopes by radiographic film or ...
For many years the use of radiolabels was the method of choice for nucleic acid labelling and detection. More recently,however,considerable research has lead to the development of a comprehensive ...
2-D Electrophoresis, Principles and Methods