PlasmidSelect Xtra platform - for purifying high-quality plasmid DNA and analyzing quantity and quality in complex solutions

PlasmidSelect Xtra chromatography medium forms the basis of a generic process for purifying supercoiled (sc) covalently-closed circular plasmid DNA from open circular (oc). The process is scalable, robust, and economic and will help meet emerging market needs for gene therapy and DNA vaccination products. The same principle can be used to rapidly analyze the quantity and quality of plasmid DNA in complex solutions. PlasmidSelect Xtra platform: Generic process for purifying supercoiled plasmid DNA Consistent from research to cGMP manufacturing PlasmidSelect Xtra Screening Kit: Quick and easy analysis with an ÄKTAdesign™ system PasmidSelect Xtra Starter Kit: Prepacked columns for convenient process development Bulk medium: PlasmidSelect Xtra is a BioProcess™ medium available in large quantities for scale-up and manufacture The PlasmidSelect Xtra process comprises RNA removal and buffer exchange on Sepharose™ 6 Fast Flow, capture and selective desorption of sc plasmid DNA on PlasmidSelect Xtra, and final polishing on SOURCE™ 30Q. The complete process comprises both chromatography and filtration, for example, clarification and ultrafiltration before and after the three chromatography steps. The HiPrep™ and HiTrap™ prepacked columns of the PlasmidSelect Xtra Starter Kit facilitate process optimization and verification. PlasmidSelect Xtra Screening Kit, which contains HiTrap™ Sepharose™ HP and HiTrap PlasmidSelect Xtra columns, quickly and accurately determines plasmid DNA quantity and quality (ratio sc/oc plasmid DNA) during fermentation and process development. For more information, please download the PlasmidSelect Xtra, PlasmidSelect Xtra Starter Kit and PlasmidSelect Xtra Screening Kit Data File, Fast determination of plasmid DNA quantity and quality in complex solutions application note, and PlasmidSelect Xtra for downstream processing of supercoiled plasmid DNA application note.