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 DeCyder Differential Analysis Software
DeCyder 2-D startup screen showing the analysis workflow
DeCyder v6.5 Differential Analysis Software Gives You Fully Automated Differential Analysis With Statistical Confidence
DeCyder™ v6.5 Differential Analysis Software has been specifically developed as a key element of the Ettan™ DIGE system. This proprietary software significantly increases throughput by accurately addressing measurement of protein differences with statistical confidence and reducing hands-on time from days to minutes with minimal user-to-user variation. DeCyder Differential Analysis Software automatically detects, matches and analyzes protein spots in multiplexed fluorescent images, and is able to give routine detection of < 10% differences with > 95 % confidence. Statistical analysis is carried out on each and every difference. All within minutes.
DeCyder v6.5 Differential Analysis Software consists of several modules:
- Image Loader—loading images into an Oracle database
- Administration Tools—database administration, maintenance, and user access control
- Batch Processor—automated detection, quantitation matching, and comparison of multiple 2-D DIGE gels.
- Differential In-gel Analysis (DIA)—co-detection, background subtraction, normalization, and quantitation of spots in an image pair
- Biological Variation Analysis (BVA)—matching multiple gels for comparison and statistical analysis of protein abundance changes
- Extended Data Analysis (EDA)—providing advanced statistical analysis for characterization and classification of biological samples based on protein expression data
- XML Toolbox—exporting spot data from DIA or BVA modules for further downstream analysis
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Differential In-gel Analysis (DIA)
The DIA software module provides consistent and accurate co-detection of image pairs from the same gel using novel algorithms. Background subtraction, quantitation, normalization and first level matching within gel are automated for high-throughput analysis with low experimental variation.
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- Co-detection of 3 images (2 samples and 1 internal standard)
- Automated detection, background subtraction, quantitation, normalization and first level (in-gel) matching
- Low user interaction, high throughput
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- DeCyder v6.5 offers a new GUI interface with improved spot detection, ability to exclude areas and spot dialog
- Very low experimental variation
- Optimized workflow for 2-D DIGE analysis
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Biological Variation Analysis (BVA)
Using batch-processing, up to several hundred gel pair-images can be processed unattended for significant time savings. Images are then matched between gels using the BVA software module that looks for consistency of differences between samples across all the gels and applies statistics to associate a level of confidence for each of those differences. Finally, the XML Toolbox can be used to export spot data from DIA or BVA for downstream analysis.
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- Automated gel-to-gel matching
- Statistical / Trend analysis using Student's t-test, One-way and Two-way ANOVA analysis
- DeCyder v6.5 has improved matching algorithms, sorting of multiple image views, and improved spot information.
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- The NCBI protein database is included as the default databse
Internal standardization
Appropriate Experimental Design and Statistical Analysis (EDSA) is central to 2-D DIGE technology and ensures the best possible results. The recommended EDSA utilizes the internal standard already described in the CyDye DIGE Fluors section, to eliminate gel-to-gel variation.
Automatic statistical analysis
2-D DIGE is the only electrophoresis technology that enables internal standardization of differences in protein abundance levels. DeCyder Differential Analysis Software has an EDSA setting feature for fully automated statistical analysis. The DeCyder Differential Analysis Software automatically generates a list of statistically significant differences, based on the threshold parameters determined by the user.
DeCyder Differential Analysis Software: the facts
- Automatic analysis: significantly reduces hands-'on time
- No image editing, results are objective
- No spot matching within gels, eliminating matching errors
- Automatic presentation of spot statistics
- Internal standard approach, increasing accuracy and simplifying gel-to-gel matching
- Automatic Oracle™ client and server installation, offering easy project handling and data security
- Optional DeCyder Extended Data Analyzer (EDA), a multivariate statistics module for easier and faster biomarker discovery
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Technical Specifications
DeCyder 2-D Differential Analysis Software v 6.5 (PDF)
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