.. | Protein labelling with novel CyDye™ DIGE Fluor minimal dyes. |
.. | Separation by 2-D electrophoresis up to three samples (one of which is the internal pooled standard) can be simultaneously separated on a single 2-D gel, using IPGphor™ System or Multiphor™ II System in the first dimension and the Ettan DALT System in the second dimension. |
.. | Advanced imaging using the Typhoon™ 9400 Variable Mode Imager. The gel is scanned using the highly sensitive Typhoon 9400 Variable Mode Imager, optimized for the Ettan DIGE System. Perpendicular scanning assures uniform images, which allows for accurate quantification of protein expression levels and identification of spots with high precision. |
.. | Analysis with DeCyder™ Differential Analysis Software developed specifically for the Ettan DIGE System to accurately measure very small protein differences with high confidence. DeCyder Differential Analysis Software automatically locates and analyzes multiplexed samples in a gel within minutes. It allows complex analysis of multiple gels to provide comparative analysis and accurate measurement of differential protein expression. |
.. | Automated spot picking Proteins of interest can be automatically picked out of the 2-D gel using Ettan Spot Picker and transferred to microtiter plates. |
.. | Automated spot digestion The proteins are then processed to enable identification using Ettan Digester.
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 | Automated MALDI spotting Digested samples are transferred to MALDI-ToF MS sample trays using Ettan Spotter. Samples are mixed with matrix in preparation for MALDI-ToF MS analysis |
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