Choose the right purification media
Ni Sepharose™ 6 Fast Flow (FF) medium is ideal for:
1. Manual purification such as gravity-flow and batch purification
2. Expression screening in multiwell plates
3. Higher flow rates purification with a laboratory pump or chromatography system such as ÄKTAdesign™ systems from GE Healthcare
4. Easy scale-up
Ni Sepharose™ High Performance (HP) medium is designed to give:
1. More concentrated target protein
2. High performance purification
It is preferably used with a laboratory pump or a chromatography system such as ÄKTAdesign systems from GE Healthcare. |
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Simply break and run – Pure histidine-tagged proteins in 30 minutes – from unclarified lysate
- One-step purification from unclarified lysates, without centrifugation and filtration
- Sample preparation time is shortened, minimizing degradation and improving the yield of active target protein
NEW formats of Ni Sepharose™ for purification of histidine-tagged proteins directly from unclarified lysates include:
- His GraviTrap™ prepacked columns for gravity flow purification – an easy way to get pure protein without use of equipment
Histidine-tagged protein purification with the highest binding capacity on the market
Looking for high quality, high performance, and reliable results? GE Healthcare offers a broad variety of pre-packed columns, kits and bulk purification media for Histidine-tagged protein purification, from simple gravity-flow and batch purification up to the fully automated high-throughput applications on the ÄKTAxpress™ system. We are a world leader in supplying complete protein purification solutions to academic and industrial customers.
Ni Sepharose™ offer:
- Pre-charging with Nickel
- Dramatically higher purification yields
- Minimized risk of inactive target protein
- Greater operational flexibility & reduced hands-on time
This is achieved by:
- Highest binding capacity on the market today (up to 4 times)
- Negligible leakage of the Ni2+ ions
- Excellent compatibility with a very wide range of reducing agents, detergents and other additives
- Convenient pre-packed columns and bulk packs
Higher binding capacity and yield mean better value and better results
The excellent binding capacity of Ni Sepharose allows for less purification media and buffers to be used, saving you time and money.
Customer feedback on Ni Sepharose 6 Fast Flow.
Consult the product selection guide to select the best product for your Histidine-tagged protein purification needs.
New to Histidine-tagged protein purification? Get started with the basics.
Find out how our his-tagged protein purification products compare to other suppliers.
See our excellent track record as a supplier for the research community and biopharmaceuticals manufacturing processes. | 
