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SELECTION GUIDE – Precision Columns
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| Technique/Column | Dimensions | Vol (ml) | Particle Size (”m) | Practical Loading Range (”g) | Detection Limit (single peak) | Flow Rate (ml/min) | pH Stability (Long term; Short term) | OPL**
(MPa) |  |
| Reversed Phase | | | | | | | | | |
| ”RPC C2/C18, SC 2.1/10* | 2.1 x 100 | 0.35 | 3 | <= 500 | <=1 ng | 0.01-0.25 | 2-8; 2-8 | 25 | |
| Ion Exchange | | | | | | | | | |
| Mono Q PC 1.6/5 | 1.6 x 50 | 0.10 | 10±0.5 | <= 500 | 10ng/280 nm, < 10 ng/214 nm | 0.01-0.4 | 2-12; 2-14 | 5 | |
| Mono S PC 1.6/5 | 1.6 x 50 | 0.10 | 10±0.5 | <= 500 | 10 ng/280 nm,
< 10 ng/214 nm | 0.01-0.4 | 2-12; 2-14 | 5 | |
| Mini Q PC 3.2/3 | 3.2 x 30 | 0.24 | 3 | <= 200 | <=1 ng | <=1 | 3-11; 1-14 | 10 | |
| Mini S PC 3.2/3 | 3.2 x 30 | 0.24 | 3 | <= 200 | <=1 ng | <=1 | 3-11; 1-14 | 10 | |
| Gel Filtration | | | | | | | | | Separation Range (Mr ) |
| Superose 12, PC 3.2/30 | 3.2 x 300 | 2.4 | 10 | <= 500 | 10 ng/280 nm,
1 ng/214 nm | 0.01-0.10 | 3-12; 1-14 | 2.4 | 1 000-300 000 |
| Superose 6, PC 3.2/30 | 3.2 x 300 | 2.4 | =13 | <= 500 | 10 ng /280 nm,
1 ng / 214 nm | 0.01-0.10 | 3-12; 1-14 | 1.2 | 5 000 - 5 000 000 |
| Superdex Peptide, PC 3.2/30 | 3.2 x 300 | 2.4 | =13 | <= 500 | 10 ng/280 nm,
1 ng/214 nm | 0.01-0.15 | 1-14; 1-14 | 2.0 | 100-7 000 |
| Superdex 75, PC 3.2/30 | 3.2 x 300 | 2.4 | =13 | <= 500 | 10 ng/280 nm,
1 ng/214 nm | 0.01-0.10 | 3-12; 1-14 | 2.4 | 3 000 - 70 000 |
| Superdex 200, PC 3.2/30 | 3.2 x 300 | 2.4 | =13 | <= 500 | 10 ng/280 nm,
1 ng/214 nm | 0.01-0.10 | 3-12; 1-14 | 1.5 | 10 000 - 600 000 |
* ”RPC is a microparticulate RPC matrix, based on a highly porous, spherical silica with a particle size of 3 ”m and an average pore size of 120Ć, covalently bonded to C2/C18 alkyl chains.
**OPL: Operational Pressure Limit |
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