GPCR second messengers — HitHunter EFC assays
Enzyme Fragment Complementation for cAMP or cGMP detectionHitHunter cAMP and cGMP Assays utilize an Enzyme Donor (ED) peptide conjugated to either cAMP or cGMP. This Enzyme Donor (ED) fragment is capable of reassociation with the enzyme acceptor (EA) to form an active beta-galactosidase (ß-gal), but it is also recognized by an anti-cAMP / anti-cGMP antibody.
cAMP EFC Chemiluminescence HitHunter Assays |
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| Suspension cells | X | X | X | | X |
| Adherent cells | X | X | | X | |
| Tissue preps | | | | X | X |
| S:B ratio | >3 | >30 | >7 | >4 | >4 |
| PDE Assays | | X | | | |
| 384-well format | X | X | X | X | X |
| 1536-well format | | | X | | |
| Sensitivity | ++++ | ++ | ++ | + | ++ |
| Prep time | 4 h | 4 h | 4 h | 4 h | 5 h |
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In the assay, the anti-cAMP / anti-cGMP antibody is optimally titrated to bind the Enzyme Donor (ED) conjugate and inhibit the beta-galactosidase formation. It also enables the measurement of competing intracellular cAMP / cGMP and the impact of other ligands and binding proteins on the complementation pathway.
Product selection guide — Select the best high throughput screening assay for your needs
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